PROGRAM STRUCTURE
Listed below please find the syllabus and course schedule
The Syllabus
A. Basic theoretical aspects
1. Research in life sciences: An overview and a few specific examples.
2. Safety, common equipment and reagents used; basics of computer and statistics.
3. Introduction to bioethics, IPR issues, some of industrial activities and practices and related statutory regulations.
B. Practical sessions: Regular assignments
1. Record keeping (patent-oriented) and other relevant professional skills (e.g., presentation skills, literature search, experimental designs, interpretation of results, and team work).
2. Understanding accuracy, precision and variations in results.
3. Basic bioinformatics: Sequence analysis, primer designing and related databases and tools.
C. Practical sessions with repeated performance by the students
1. Basic bacterial culture methods.
2. DNA and RNA handling:
Genomic DNA (plant, microbial and animal) isolation and quantification
Analysis of DNA samples by gel electrophoresis
RNA isolation
Quantification and gel electrophoresis of RNA
3. Recombinant DNA technology
Primer designing and PCR
Restriction digestion and ligation
Preparing competent cells and transformation
Cloning and plasmid isolation
4. Protein handling
Extraction and quantification
Native and SDS-PAGE; staining procedures for the same.
D. Practical sessions with limited performance by students
Purification of DNA after enzymatic reactions
mRNA isolation
Reverse transcription-PCR
Blotting DNA and RNA for Southern and Northern
Two-dimensional electrophoresis
Expression of cloned sequences
Western and ELISA
Purification: Chromatography
Mammalian cell culture and transfection
E. Demonstrations/theory only
Complete Southern and Northern procedures
DNA sequencing and Microarrays
Protein activity assays
F. Networking and exposure
Visits to industrial/academic organizations and interactions with eminent persons in the related area.
G. Project work in a specialized area
The work might involve one or more of the above mentioned techniques.
Course schedule
Last date for submitting filled application forms: 09 August 2006.
The application form fee is non-refundable. Forms received after the last date will be rejected.
Tentative dates for selection procedures: Test and Interview on 20 Aug. and 14 Sept., 2006, respectively.
Course Duration
6 months (certificate course) or 12 months (diploma, with project work).
Course Commences: Tentatively in the first week of November 2006.
Maximum no. of seats: 20