DR. SHIPRA AGRAWAL

	CURRENT RESEARCH ACTIVITIES
	1.  Web based databases: Developing web based specialized public databases on various important chromatin associated/binding proteins.
	Chromatin remodeling is an important event in the eukaryotic nucleus rendering nucleosomal DNA accessible for various transaction processes. The dynamic nature of chromatin is facilitated by Remodeling Factors through participation of the collective action of (a) ATP and (b) Non-ATP dependent factors. Considering the importance of these factors in mammals and eukaryotes, we have developed a specialized web database CREMOFAC , a dedicated and frequently updated web-database for chromatin remodeling factors. The database contains factors from 49 different organisms reported in the literature and facilitates a comprehensive search for them. In addition, it also provides in-depth information for the factors reported in the three widely studied mammals namely, human, mouse and rat. Further, information on the literature, pathways, and phylogenetic relationships has also been included.
	2.  Web based tools
	(i) We are working on developing  a tool, which can facilitate housing and pathway based analysis of   microarray expression data of several metabolic genetic disorders reported in human and animal models. The tool may also help in identifying new genes involved in diseases and expression signature(s) associated with each of these diseases.
	(ii) We are also working on developing a tool to perform genome wide mapping of micro-RNA in higher eukaryotes with higher sensitivity and efficiency
	MicroRNAs (miRNAs) are small (range 19-25 nt), single stranded RNAs that form imperfect duplexes with the 3'UTR regions of target messenger RNAs. miRNAs are transcribed as short hairpin precursors and are processed into active miRNAs by Dicer, a ribonuclease.
	It takes part in post-transcriptional regulation either by arresting the translation of messenger (m) RNAs or by cleaving the mRNAs. It has also been shown that miRNAs cause repression of a number of tumor suppressors; thereby leading to cancer development and progression (Violinia et al.2006). Hence, genome wide mapping miRNAs and their targets is important in order to understand the miRNAs controlled regulation of gene expression in different organisms.
	We are considering all possible conserved features associated with precursor and mature miRNAs to develop a machine learning approach to map the candidate miRNAs in a given genome.
	3. Identification of conserved sequence motifs associated with human meiotic  recombination hotspots. Novel signature motifs have been identified across more than 7,000 hotspots. Further, these motifs have been shown to be significantly related with the recombination activity of these hotspots
	Tools Developed
	PirnaBank: A web resource on classified and clustered piRNAs T2D-db: An integrated data resource on Type 2 Diabetes CREMOFAC: A web-database of Chromatin Remodeling Factors
	PATENTS
	U.S. Patent. No. 6,620,599 B1 Date Sep. 16, 2003 TITLE: Process for the Production of a biological Active Phenolic Compound (+) Catechin
	U.S. Patent. No. 6,365,757 B1 Date Apr. 2, 2002 TITLE: Process for the Production of a Compound (+) Catechin PentaAcetate Useful as a  Precursor for the Production of (+) Catechin
	UK Patent. No. GB 2360 516 Date 26.09.2001 .   TITLE: Preparation of (+) Catechin
	Canadian Patent No. 2303 550 A1 Date 2001/09/30 TITLE: : A Process for the Production of a Stable (+) Catechin PentaAcetate Useful as a  Precursor for the Production of (+) Catechin
	PUBLICATIONS
	Year 2010
	Analysis of Meiotic Recombination Hotspots: A Bioinformatics Approach. Agrawal Shipra, Nishant K.T. and M.R.S. Rao. Book Chapter (In Press) Biology of Aurora A kinase: Implications in cancer manifestation and therapy. Karthigeyan D, Prasad SB, Shandilya J, Agrawal Shipra, Kundu TK. Med Res Rev. 2010 Mar 1. Ahead of print Identification of a novel inhibitor of coactivator-associated arginine methyltransferase 1 (CARM1)-mediated methylation of histone H3 Arg-17. Selvi BR, Batta K, Kishore AH, Mantelingu K, Varier RA, Balasubramanyam K, Pradhan SK, Dasgupta D, Sriram S, Agrawal Shipra, Kundu TK. J Biol Chem. 2010 Mar 5; 285(10):7143-52.
	Year 2009
	1. Expression based network biology identifies alteration in key regulatory pathways of Type 2 Diabetes and associated risk / complications. Urmi Sengupta, Sanchaita Ukil, Nevenka Dimitrova and Shipra Agrawal. PLoS ONE 4 (12) e8100 (2009) 2. Inhibition of Lysine Acetyltransferase KAT3B/p300 Activity by a Naturally Occurring Hydroxynaphthoquinone, Plumbagin.Ravindra KC, Selvi BR, Arif M, Reddy BA, Thanuja GR, Agrawal Shipra, Pradhan SK, Nagashayana N, Dasgupta D, Kundu TK. J Biol Chem. 2009 Sep 4;284(36):24453-64. 3. Mechanism of p300 specific histone acetyltransferase inhibition by small molecules.Arif M, Pradhan SK, Thanuja GR, Vedamurthy BM, Agrawal Shipra, Dasgupta D, Kundu TK. J Med Chem. 2009 Jan 22;52(2):267-77. 4. Identification of a novel inhibitor of CARM1-mediated methylation of histone H3R17. Selvi BR, Batta K, Kishore AH, Mantelingu K, Varier RA, Balasubramanyam K, Pradhan SK, Dasgupta D, Sriram S, Agrawal Shipra, Kundu TK. J Biol Chem. 2009 Dec 17. [Epub ahead of print]
	Year 2008
	Agrawal, S., Dimitrova, N., Nathan, P., Udaykumar, K., Sailakshmi, S, Sriram, S, Nair, M., Sengupta, S. T2D-Db: An integrated platform to study the molecular basis of Type 2 diabetes. BMC Genomics, 9, 320 (2008)
	Sai Lakshmi, S. and Shipra Agrawal. piRNABank: A web resource on classified and clustered piwi interacting RNAs. Nucleic Acids Research 36: D173-7 (2008).
	Kishore H, Vedamurthy BM, Mantelingu K, Shipra Agrawal, Reddy BA, Roy S, Rangappa K, Kundu TK.  Specific small-molecule activator of aurora kinase a induces autophosphorylation in a cell-free system. J Med Chem. 28; 51 (4):792-7(2008).
	Year 2007
	M M Pradeepa , S Manjunatha , V Sathish , Shipra Agrawal , M R S Rao.   Involvement of Importin4 in the Transport of Transition protein 2 into Spermatid Nucleus. Mol Cell Biol. 2007 Aug 6: 17682055.
	Kishore H, K Batta, C Das, Shipra Agarwal and Tapas K. Kundu p53 regulates its own activator: transcriptional co-activator PC4, a new p53-responsive gene. Biochem. J. (2007) 406 (437–444).
	Year 2006
	Agrawal, S., Kumar, C. and Rao, M.R.S. CREMOFAC: A database of chromatin remodeling factors. Accepted (Bioinformatics). DOI: http://bioinformatics.oxfordjournals.org/cgi/content/abstract/btl509?
	Kishore,H., Batta,K., Das,C., Agrawal, S. and Kundu, T..   P53 regulates its own activator-A new P53 responsive gene. In press (Nucleic Acid Research)
	Nishant,K. T., Agrawal, S., Kumar,C., Sathish, V. and Rao,M.R.S. Bioinformatic analysis of human meiotic recombination hot spots. Presented at the "Gordon Research Conference'06", London
	Year 2005
	Vijayasri, S. and Agrawal, S. Domain based modelling and conformational epitope mapping of coat protein of West Nile Virus. Journal of Molecular Modelling 11: 248-255 (2005)
	Khan, F., Agrawal, S. and. Mishra B.N. Genome wide identification of DNA binding motifs of NodD- factor in Sinorhizobium meliloti and Mesorhizobium loti. Journal of Computational biology and Bioinformatics. 773-801 (2005)
	Khan, F., Agrawal, S. and Mishra, B.N. In silico identification of cis-regulatory elements in Mesorhizobium ioti. Online Journal of Bioinformatics 6, 129-141 (2005)
	Year 2004
	Gupta A and Agrawal, S. Wave analysis of microarray expression as predictor of gene expression in human tumors, On line Journal of Bioinformatics. 5: 49-58. (2004)
	Agrawal, S., Kumar, A., Srivastava, V. and Mishra, B.N. Cloning, Expression, Activity and Folding Studies of Serine Hydroxymethyl Transferase: A Target Enzyme for Cancer Chemotherapy.  Journal of Molecular Microbiology and Biotechnology. 6:67-75. (2004)
	Singh, I.V., Agrawal, S., Khan, F., Mishra B. N. Phylogenetic Clustering and Secondary structural analysis of Plant Terpenoid   Synthases. On line Journal of Bioinformatic, 5: 32-48. (2004)
	Year 2003
	Khan, F., Agrawal, S. and Mishra, B. N. Identification of GltC transcription factor binding DNA motifs and its novel co-regulated genes in nitrogen fixing bacteria. Online Journal of Bioinformatics. 7:106-114. (2003)
	Agrawal, S., Bannerjee, S, Chattopadhyay, S.K., Shashidhar, K.V. and Kumar, S. Synthesis of (+) Catechin penta – acetate by a cell culture of Himalayan yew, Taxus wallichiana. Indian Journal of Biotechnology 2:264-267. (2003)
	Year 2001
	Mishra, B.N., Agrawal, S. and Khare, A.K. Mathematical modeling of axial and recycle back mixing in a biofilm anaerobic fixed bed reactor. Different disorders: System: Indias Publication 320-322 (2001)
	Year 2000
	Agrawal, S., Bannerjee, S, Chattopadhyay, Kulshreshtha, M., Madhusudanan, K.P., Mehta, V.K. . and Kumar, S. Isolation of Taxoids from Cell Suspension Cultures of Taxus  wallichiana Planta Medica . 66(8): 773-775. (2000)
	Agrawal, S., Bannerjee, S, Kulshreshtha, M., Chattopadhyay, S.K., Gupta, M. M. and Kumar, S. Production of (+) catechin in the callus cultures of Himalayan yew, Taxus wallichiana JAMAPS, 22/4A, 244-247 (2000)
	Agrawal, S., Kumar, A., Banerjee, S., Gupta, M.M., Verma, R.K., Singh, D.V.and Kumar, S. Production of biloblide in cultures of clone GBC –1 of Ginkgo biloba JAMAP: 22/4A, 194 - 196. (2000)
	Book Chapter
	Agrawal, S. and Mishra, B.N. Bioinformatics studies in plant systems A book Chapter in Plant Genetic Engineering – A Multivolume Series, Vol. 1, 2002, SCI-TECH. PUBLISHING LLC, 9207, Country Creek Drive, Houston, Texas-77036 (USA)
	CONFERENCES ATTENDED
	Agrawal, S., Mishra, B.N., Yadav, D.S., Chandra,G., Goyal, R., Mishra, S., Khare, A.K., and Agrawal, R. (2003). Recent trends in drug design: A bioinformatics approach. Vol. 1, 44-53, Workshop on Computer Science and Information Technolology, CSIT’2003, UFA, Russia, Proceedings of the 5th workshop, UFA, Russia.
	Ph.D.
	(Biotechnology) Center for Aromatic and Medicinal Plants (CIMAP) Council for Scientific and Industrial Research, Lucknow (April 2001)