- Developing tuneable yeast gene expression system using synthetic promoters and gene editing technology
Distinct biotechnological applications require different promoter properties in terms of expression range and inducibility. Currently promoter discovery is largely focused on manipulating native promoters which are large DNA sequences and are regulated in a complex fashion. We are generating a library of yeast cells containing distinct promoter activities for production of high value biomolecules over a broad dynamic range in a high throughput format. To achieve this, the group is focusing on engineering novel synthetic promoters that are tuneable, programmable and orthogonal in nature. In addition, we are developing a repository of synthetic genetic parts and elements that together with the promoters will create a suite of expression cassettes for customized and enhanced expression of biomolecules.
- Metabolic engineering of yeast cells for biotechnological applications
Yeast is recognized as GRAS organism and is used extensively for industrial production of valuable molecules. In this project, we will be generating value-added yeast strains that overexpress phytochemicals, food supplements, human metabolic enzymes and nutraceuticals. This will be achieved by rational engineering of the endogenous metabolic genes in yeast cells or transplanting genes from heterologous sources by using recombinant DNA methods. Using gene editing technologies, we also seek to modify metabolic pathways in yeast cells to generate “humanised yeast stains” for production of human therapeutic proteins and biologics.
- Engineering human cells for therapeutic applications
We are establishing a robust protocol and platform for precise bespoke gene editing of human cells for various therapeutic purposes. The group will be generating human cell lines that (a) mirror disease-causing mutations for drug resistance studies and screening purposes, (b) develop gene editing strategies for gene correction and functional genomics studies, and (c) improve existing therapeutic approaches to autoimmune diseases, cancers and rare genetic disorders.
- Functional genomic approaches to combat vector-borne diseases
Recently viruses belonging to the family Flaviviridae have become a major public health problem worldwide including India. We will focus on Aedes-Dengue virus infection model to identify and characterize key factors controlling virus multiplication in the mosquito using genome wide approaches. Subsequently we aim to translate this knowledge to design effective disease transmission blocking strategies and extend to other viral infections.
PhD at Indian Institute of Chemical Biology (Jadavpur University), Kolkata
Lecturer at Birla Institute of Technology, Mesra
Postdoctoral training at EMBL Heidelberg, McGill University, Oxford University and Cambridge University
Faculty at IBAB since 2017
Areas of Expertise and Interests:
Synthetic Biology, Genome Engineering Technologies, Functional Genomics, Host-parasite Interactions, Coding & Non-coding RNA Functions, Medical Biotechnology, Biosensors
Awards and Distinctions:
Ramalingaswamy fellowship, Department of Biotechnology
Research recognition award, University of Oxford, UK
Travel grant award, Cold Spring Harbor Meeting, USA
Research Fellowship from University Grants Commission during PhD
Qualified National Eligibility Test for Lecturership and Research Fellowship
MSc merit scholarship from University Grants Commission, Government of India
Niranjan Veluru (Postdoctoral Fellow)
Niranjan completed his PhD research project on flagellar biogenesis in Leishmania at Central Drug Research Institute, Lucknow. Subsequently, he worked on membrane transport and ER stress regulated pathological diseases at Indian Institute of Science, Bengaluru and joined the group in February 2018. His current research interests include structure-functional analyses of proteins and developing efficient gene editing methods in human and insect cells.
Poornima R (Project Assistant)
Poornima completed her masters in Biotechnology from Kuvempu University, Karnataka and worked at Biocon Research Centre, Bengaluru as a research trainee before joining the group in September 2017. She is currently working on generating yeast strains with synthetic promoter containing cassettes for enhanced expression of biomolecules.
Anusha KR (Project Assistant)
Anusha completed her M.Tech in Molecular Medicine from Amrita Centre for Nanosciences and Molecular Medicine, Amrita Vishwa Vidyapeetham, Kochi and joined the group in February 2018. She is currently using gene editing technologies to modify human cells for therapeutic applications.
Bilal Akhtar (Trainee, MSc IBAB)
Mansi Srivastava (Trainee, MSc IBAB)
Pritha Dey (Trainee, MSc IBAB)
Tanisha Gupta (Trainee, MSc IBAB)
Tanushree Sengupta (Trainee, MSc IBAB)
Usha TM (Trainee, MSc Bangalore University)
List of Publications:
- Ghosh, S.* and Liu, J.L.* (2018) Genomic Tagging of AGO1 using CRISPR/Cas9-mediated Homologous Recombination. Methods in Molecular Biology, 1680: 217-235.
- Yong, H., Wang, J.J., Ghosh, S.* and Liu, J.L.* (2017) Critical roles of CTP synthase N-terminal in cytoophidium assembly. Experimental Cell Research 354(2):122-133.
- Ghosh, S. *, Tibbit, C., and Liu, J.L.* (2016) Efficient knockdown of Drosophila long non-coding RNAs by CRISPR interference. Nucleic Acids Research, 44(9): e84.
- Ghosh, S.* and Lasko, P.* (2015) Loss-of-function analysis reveals distinct requirements of the translation initiation factors eIF4E, eIF4E-3, eIF4G and eIF4G2 in Drosophila spermatogenesis. PLOS One 10(4): e0122519.
- Ghosh, S., Obrdlik, A., Marchand, V. and Ephrussi, A. (2014) The EJC binding and dissociation activity of PYM is regulated in Drosophila. PLOS Genetics 10(6): e1004455.
- Ghosh, S.*, Marchand, V.*, Gaspar, I. and Ephrussi, A. (2012) Control of RNP motility and localization by a splicing-dependent structure in oskar mRNA. Nature Structural Molecular Biology 19(4), 441-449.
- Ghosh, S., Goswami, S. and Adhya, S. (2003) Role of superoxide dismutase in survival of Leishmania within the macrophage. Biochemical Journal 369(Pt.3), 447-452.
- Adhya, S., Basu, S., Bhattacharyya, S.N., Chatterjee, S., Dhar, G., Goswami, S., Ghosh, S., Home, P., Mahata, B. and Tripathi, G. (2003) Mitochondrial differentiation in kinetoplastidprotozoa: a plethora of RNA controls. Differentiation 71, 549–556.
KBITS, Department of Innovation and Technology, Government of Karnataka
Department of Biotechnology, Government of India
The Synthetic Biology group is open to receiving strong applications for Postdoc, PhD and Project Assistant positions. We welcome highly motivated team players in our group who can think out of the box, learn new techniques and enjoy solving problems. Candidates with relevant experience and fellowships will be preferred.
Interested applicants may send a cover letter outlining their motivation and a CV showing the skills and expertise as a single pdf file to Dr. Sanjay Ghosh
Dr. Sanjay Ghosh
Synthetic Biology Group
Institute of Bioinformatics and Applied Biotechnology
Biotech Park, Electronic City Phase I
Bengaluru – 560100
Phone: +91 (080) 28528901/902/903 (extn 209)