Research Focus Key Words
Synthetic biology,Metabolic engineering, Genome Engineering Technologies, Functional Genomics, Host-parasite Interactions
Dr. Ghosh completed his Masters in Zoology from Banaras Hindu University and received his PhD degree from Jadavpur University (IICB, Kolkata). Subsequently, he joined BIT Mesra as Lecturer in Biotechnology. For his postdoctoral training, Dr. Ghosh worked at European Molecular Biology Laboratory, Heidelberg and McGill University on RNA transport and translation mechanisms in Drosophila. At University of Oxford, he demonstrated the use of CRISPR interference method to knock down long non-coding RNAs. Thereafter, he worked at the University of Cambridge on genome wide stress-dependent translation response in yeast using ribosome profiling. He is the recipient of Ramalingaswami re-entry fellowship of the Department of Biotechnology for the year 2016-2017.
- PhD, Indian Institute of Chemical Biology (Jadavpur University), Kolkata
- Postdoctoral training at EMBL Heidelberg, McGill University, University of Oxford and University of Cambridge
- Lecturer, Birla Institute of Technology Mesra, Ranchi
- Faculty at IBAB since 2017
We are primarily interested in developing translatable technologies and products using synthetic biology approaches. We utilize latest genome editing technologies and DNA assembly methods to engineer microorganisms and human cells.
- Developing tunable yeast gene expression system using synthetic promoters and gene editing technology
Distinct biotechnological applications require different promoter properties in terms of expression range and inducibility. Currently promoter discovery is largely focused on manipulating native promoters which are large DNA sequences and are regulated in a complex fashion. We are generating a library of yeast cells containing distinct promoter activities for production of high value biomolecules over a broad dynamic range in a high throughput format. To achieve this, the group is focusing on engineering novel synthetic promoters that are tuneable, programmable and orthogonal in nature. In addition, we are developing a repository of synthetic genetic parts and elements that together with the promoters will create a suite of expression cassettes for customized and enhanced expression of biomolecules.
- Metabolic engineering of yeast cells for biotechnological applications
Yeast is recognized as GRAS organism and used extensively for industrial production of valuable molecules. In this project, we are generating value-added yeast strains that overexpress phytochemicals, food supplements, human metabolic enzymes and nutraceuticals. This will be achieved by rational engineering of the endogenous metabolic genes in yeast cells or transplanting genes from heterologous sources by using recombinant DNA methods. Using gene editing technologies, we also seek to modify metabolic pathways in yeast cells to generate “humanised yeast stains” for production of human therapeutic proteins and biologics.
- Engineering human cells for therapeutic applications
We are establishing robust protocols and platform for precise bespoke gene editing of human cells for various therapeutic purposes. The group will be generating human cell lines (a) to develop gene editing strategies for gene correction and functional genomics studies, (b) to improve existing therapeutic approaches to autoimmune diseases, cancers and rare genetic disorders, and (c)that mirror disease-causing mutations for drug resistance studies and screening purposes.
- Functional genomic approaches to combat vector-borne diseases
Recently viruses belonging to the family Flaviviridae have become a major public health problem worldwide including India. Using the Aedes-Dengue virus infection model, we aim to identify and characterize key factors controlling virus multiplication in the mosquito using genome wide approaches. Subsequently we will be translating this knowledge to design effective disease transmission blocking strategies for Dengue and other viral infections.
- Shubham Dixit
Shubham completed Bachelor’s and Master’s degree in Biotechnology from Banasthali University, Rajasthan. She worked at ICGEB and NIPGR New Delhi and carried out her PhD research project in plant molecular biology and physiology at Banasthali University. Shubham joined IBAB in 2015 as postdoc to work on the Amaranth Genome project. Thereafter, she joined Synthetic biology group in 2018 and is involved in developing yeast strains producing high value phytochemicals, metabolites and therapeutic enzymes.
- Aparna MS
Aparna completed her Masters Engineering in Biotechnology from Birla Institute of Science and Technology, Pilani, Rajasthan. She worked in the Structure Based Drug Design department at Aurigene Discovery Technologies for 10 years as Senior Scientist, Protein Sciences. Aparna joined the group in 2018 and is working on projects that involve improving efficacy of therapeutic enzymes as well as structure-function analyses of DNA repair enzymes.
- Shikha Kumari
Shikha completed Master’s degree in Biotechnology from Hemvati Nandan Bahuguna Garhwal University, Uttarakhand. She worked at Syngene International Ltd., Bengaluru as a Research Associate before joining the group in 2019. Her area of expertise is optimization, development and validation of cell-based assays. Shikha’s research interest lies in the development of antibodies for immunotherapy.
- Sara Ashok Varghese
Sara completed her Master’s in Biotechnology from Loyola College, Chennai and did summer project work in Department of Genetics at University of Delhi South Campus. She joined the lab in 2019 and is working on analyses of host-dengue virus infection using functional genomics approaches.
- Poornima R (Project Assistant)
- Anusha KR (Project Assistant)
- Niranjan Veluru (Postdoctoral Fellow)
- Deepthi Menon (Postdoctoral Fellow)
- T Sai Kavya Rao (Project Trainee)
- Anjitha P (Project Trainee)
- Bilal M Akhtar (Trainee, MSc06 IBAB)
- Mansi Srivastava (Trainee, MSc06 IBAB)
- Pritha Dey (Trainee, MSc06 IBAB)
- Tanisha Gupta (Trainee, MSc06 IBAB)
- Tanushree Sengupta (Trainee, MSc06 IBAB)
- Sneha Lahorani (Trainee, MSc07 IBAB)
- Harini N Pillai S (Trainee, MSc07 IBAB)
- Rashmi Gurudatta Sardessai (Trainee, MSc07 IBAB)
- Usha TM (Trainee, MSc Bangalore University)
- Samantha J Coelho (Research Assistant)
- Snigdha N Rao (JRF)
- Sneha C Antony (Research Assistant)
- Yamini Vadapalli (Project Assistant)
- Tata Institute for Genetics and Society, NCBS campus, Bangalore
- Acharya & BM Reddy College of Pharmacy, Bangalore
- Jananom Private Limited, Bangalore
- Syngene International Limited, Bangalore
- Indian Institute of Science, Bengaluru
Publications and Patents:
- de la Roche, M., Hamilton, C., Mortensen, R., Jeyaprakash, A.A., Ghosh, S. and Anand P.K. (2018)
Trafficking of cholesterol to the ER is required for NLRP3 inflammasome activation. Journal of Cell Biology, doi:10.1083/jcb.201709057.
- Ghosh, S.* and Liu, J.L.* (2018)
Genomic Tagging of AGO1 using CRISPR/Cas9-mediated Homologous Recombination. Methods in Molecular Biology, 1680: 217-235. *corresponding authors
- Yong, H., Wang, J.J., Ghosh, S.* and Liu, J.L.* (2017)
Critical roles of CTP synthase N-terminal in cytoophidium assembly. Experimental Cell Research 354(2):122-133. *corresponding authors
- Ghosh, S. *, Tibbit, C., and Liu, J.L.* (2016)
Efficient knockdown of Drosophila long non-coding RNAs by CRISPR interference. Nucleic Acids Research, 44(9): e84. *corresponding authors
- Ghosh, S.* and Lasko, P.* (2015)
Loss-of-function analysis reveals distinct requirements of the translation initiation factors eIF4E, eIF4E-3, eIF4G and eIF4G2 in Drosophila spermatogenesis. PLOS One 10(4): e0122519. *corresponding authors
- Ghosh, S., Obrdlik, A., Marchand, V. and Ephrussi, A. (2014)
The EJC binding and dissociation activity of PYM is regulated in Drosophila. PLOS Genetics 10(6):e1004455.
- Ghosh, S.*, Marchand, V.*, Gaspar, I. and Ephrussi, A. (2012)
Control of RNP motility and localization by a splicing-dependent structure in oskar mRNA. Nature Structural Molecular Biology 19(4), 441-449. *equal contribution
- Ghosh, S., Goswami, S. and Adhya, S. (2003)
Role of superoxide dismutase in survival of Leishmania within the macrophage. Biochemical Journal 369(Pt.3), 447-452.
- Adhya, S., Basu, S., Bhattacharyya, S.N., Chatterjee, S., Dhar, G., Goswami, S., Ghosh, S., Home, P., Mahata, B. and Tripathi, G. (2003) Mitochondrial differentiation in Kinetoplastidprotozoa: a plethora of RNA controls. Differentiation 71, 549–556.
KBITS, Department of Innovation and Technology, Government of Karnataka
Department of Biotechnology, Government of India
Biotechnology Industry Research Assistance Council (BIRAC), Government of India