Dr. Swathi Alagesan
DST-INSPIRE Faculty Fellow, Synthetic Biology
Research Focus Keywords
Synthetic biology, Flux Analysis, Metabolic Engineering
Dr. Swathi Alagesan completed Bachelors in Engineering from Delhi College of Engineering. Subsequently, she joined the Department of Chemical Engineering at IIT Bombay for a PhD. Under the guidance of Prof. Pramod Wangikar, she worked on studying the circadian rhythms in cyanobacteria. For her postdoctoral work, she joined Dr. Nigel Minton’s group at the Synthetic Biology Research Centre, University of Nottingham. During this time she was involved in generating synthetic biology parts and using them for metabolic engineering of autotrophic bacteria. In 2017, she joined Anna University as a DST-INSPIRE Faculty. She moved to IBAB in 2020 and her current research is focussed on generating various synthetic biology tools, and using the insights obtained from flux analysis to engineer microorganisms for optimizing behaviour.
- B.E. (Biotechnology) – Delhi College of Engineering, Delhi, India (2006-2010)
- Ph.D – IIT Bombay, Mumbai, India (2010-2015)
- Post-doctoral research – University of Nottingham, Nottingham, UK (2015-2017)
- DST-INSPIRE Faculty – Anna University, Chennai, India (2017-2020)
- DST-INSPIRE Faculty Scientist – IBAB, Bengaluru, India (2020- present)
In-depth understanding of the underlying metabolism of an organism is critical for rationalized metabolic engineering. Hence, in our lab, we focus on
- Understanding the underlying metabolism of micro-organisms through flux analysis – We mainly carry out 13C-based metabolic flux analysis where a 13C labelled carbon substrate is fed to the micro-organism and based on the labelling profile of metabolites within the cell, the flux through different pathways is studied. This gives key insights into the organism’s preferential metabolism under different growth conditions and also aids in rationalizing engineering strategies.
- Developing synthetic biology tools for metabolic engineering of autotrophic micro-organisms – Well characterized libraries of genetic elements are essential for building robust controllable synthetic circuits. We, at IBAB, are involved in developing such synthetic biology parts and tools for controlling gene expression in a predictable manner.
Looking for students having a research aptitude, who wish to work in the field of synthetic biology. If interested, please contact me at firstname.lastname@example.org
- Swathi Alagesan*, Erik KR Hanko*, Naglis Malys*, Muhammad Ehsaan, Klaus Winzer, Nigel P Minton
Functional genetic elements for controlling gene expression in Cupriavidus necator H16.
Appl. Environ. Microbiol., 84 (19), e00878-18, (2018) (*equal contribution)
- Swathi Alagesan, Nigel P Minton, Naglis Malys
13C-assisted metabolic flux analysis to investigate heterotrophic and mixotrophic metabolism in Cupriavidus necator H16.
Metabolomics, 14(1), 9, (2018)
- Swathi Alagesan, Sandeep B. Gaudana, Pramod P. Wangikar
Rhythmic oscillations in KaiC1 phosphorylation and ATP charge in nitrogen-fixing cyanobacterium Cyanothece sp. ATCC 51142 under continuous light.
Biological Rhythm Research, 47(2): 285-301, (2016)
- Swathi Alagesan, Sandeep B. Gaudana, Avinash Sinha, Pramod P. Wangikar
Metabolic Flux analysis of Cyanothece sp. ATCC 51142 under mixotrophic conditions.
Photosynthesis Research, 118(1-2): 191-8, (2013)
- Swathi Alagesan, Sandeep B. Gaudana, Krishnakumar S., Pramod P. Wangikar
Model based optimization of high cell density cultivation of nitrogen fixing cyanobacteria.
Bioresource Technology, 148:228-233, (2013)
- Sandeep B. Gaudana, Swathi Alagesan, Madhu Chetty, Pramod P. Wangikar
Diurnal rhythm of a unicellular diazotrophic cyanobacterium under mixotrophic conditions and elevated carbon dioxide.
Photosynthesis Research, 118: 51-7, (2013)
- Sandeep B. Gaudana, Krishnakumar S., Swathi Alagesan, Madhuri G Digmurti, Ganesh A. Viswanathan, Madhu Chetty, Pramod P. Wangikar
Rhythmic and sustained oscillations in metabolism and gene expression of Cyanothece sp. ATCC 51142 under constant light.
Frontiers in Microbiology, 4:374, (2013)